Composite

Part:BBa_K318015:Design

Designed by: Justin Vrana, Nate Pantalone   Group: iGEM10_Wisconsin-Madison   (2010-07-13)


Hin Generator (T + pLac + RBS + Hin + T + pCons + RBS + lacIq + T)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 763
    Illegal NheI site found at 786
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1964
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Constitutive promoter was placed after the hin recombinase to prevent unwanted Hin Recombinase


Source

Assembled from other biobrick parts.

References